RESOURCESRESOURCES

Community Transformation Facilities

These facilities provide the opportunity for reseaerchers to introduce their gene of interest into different model or crop species for which they might not have expertise in their host institutions.

Cornell CALS Plant Transformation Facility (PTF)

https://sips.cals.cornell.edu/research/plant-transformation-facility/

The Plant Transformation Facility (PTF) is a new service facility of the College of Agriculture and Life Sciences (CALS) and its School of Integrative Plant Science (SIPS). PTF is charged with supporting the plant biology research of Cornell faculty by making transgenic plants of time-consuming or hard-to-transform species with a particular focus on New York State crops. With the explosion of interest in gene editing technologies, much of our work involves performing CRISPR/Cas9-mediated genome modification.

 

John Innes Centre BRACT Crop Transformation Resource

https://www.jic.ac.uk/research-impact/technology-platforms/genomic-services/crop-transformation/

BRACT (Biotechnology Resources for Arable Crop Transformation) is a research facility based at the John Innes Centre providing the wider research community with access to a range of crop transformation and genome editing resources, such as: 

> Development of new targeted gene knockout resources: CRISPR/Cas9 technology

> Training facilities: hosting visitors for hands-on training at the John Innes Centre

> Constructs: developed specifically for Brassica and the Cereals – the pBRACT series of vectors

The facility operates on a ‘not-for-profit’, cost recovery basis and supports researchers worldwide.

NIAB Community Resource for Wheat and Rice Transformation

https://www.niab.com/transgenic

The Community Resource for Wheat and Rice Transformation provides capacity for 100 genes to be transformed into wheat and rice free of charge. We envisage that 75 genes will be transformed into wheat and 25 genes into rice. In the event that wheat or rice transformation is not fully subscribed, additional slots will be made available in the other species.

We will encourage researchers working with genes from model crops to test their hypotheses in wheat and rice as appropriate to the gene studied. This will enable new genes to be evaluated more quickly in crops essential to food security worldwide, and allow researchers to amass crucial data which can be used as a basis to seek follow-on funding.

ROTHAMSTED CEREAL TRANSFORMATION

https://www.rothamsted.ac.uk/cereal-transformation

Rothamsted Research offers a cereal transformation service to generate transgenic wheat plants for research purposes for both internal projects and external contracts.

Advice is available on all aspects of transformation from the design stages such as suggesting promoters to drive transgene expression or providing appropriate cloning constructs for either overexpression of genes or their down-regulation by RNAi, to assistance with crossing transgenic plants or taking transgenic lines to field trial.

A more recent addition to transformation technology is genome editing which offers the possibility to generate highly targeted mutations in the host genome. Some success has already been achieved in modifying specific target genes and the intention is to add this to the range of services offered.