The Facility for Imaging by Light Microscopy (FILM) provides access to state-of-the-art microscopy equipment and all training and assistance required for the whole range of light microscopy, from basic observations with transmitted light to multiphoton fluorescence in vitro microscopy and fluorescence lifetime imaging.
Of particular interest to plant scientists are the super-resolution microscopes, two-photon microscopy and spectral imaging/unmixing, both valuable in plants with high autofluorescence, and fluorescence lifetime imaging.
In addition to microscopy equipment, we also provide access to software and expertise in image data analysis, general microscopy education and a platform for knowledge exchange and networking. Experts at FILM share their expertise on the Protocols, Tips and Tricks page.
The Electron Microscopy (EM) Unit provides state-of-the-art EM and preparatory facilities enabling applications ranging from basic high resolution imaging of immuno-labelled samples through to frozen hydrated cryo-EM and 3D tomography.
Expert academic and general staff are available to provide support ranging from initial project discussion and planning through to training, application of techniques and assistance with data interpretation.
Users have a range of options - the Unit offers service, collaboration or training for sample prep and to use our high resolution TEM, SEM and 3View 3D-EM.
To find out more, contact: firstname.lastname@example.org
The Centre for Cell imaging (CCI) at the Centre for Integrative Biology at the University of Liverpool is a world class resource for imaging projects using optical microscopy. The CCI a strong focus on imaging dynamics events in real time on living samples, therefore all of the microscopes are fully equipped with temperature and gas control in order to maintain cells or organisms alive for several hours, even days, in their physiological environment.
The facility operates four confocal microscopes and one epifluorescence equipped for superresolution. Integrating part of the facility is the Imaging Suite, equipped with the state-of-the-art software packages for image analysis such as IMARIS, Volocity and simFCS. The CCI is open for service and collaboration and supports a wide range of techniques. The staff offers consultancy and help, from experimental planning, student and staff training to data storage and analysis.
The CCI was involved in this recent Science paper (Vermeer et al., 2014, Science 343:178).
For more information click here or contact Marco Marcello at email@example.com.
Micron Oxford is located within the Department of Biochemistry and the Dunn School of Pathology in the South Parks Road science area at the University of Oxford, and is funded by a strategic award from theWellcome Trust. It is a collaborative, multidisciplinary bioimaging unit focusing especially on the development and use of single molecule methods, light sheet and super-resolution microscopy.
OCTOPUS is at the heart of the Functional Biosystems Imaging group of the STFC Lasers for Science Facility. It brings together STFC’s core expertise in imaging, including its capabilities in in vivo single molecule fluorescence imaging, multiphoton FLIM microscopy, and Raman spectroscopy and imaging.
The aim is to make this expertise and advanced imaging technology available for collaborative research with the scientific community, through STFC’s peer-review process.
To access the gSTED microscopy instrument funded as part of BBSRC's ALERT13 programme, go to these instructions or email Marisa Martin-Fenandez. It is a Leica SP8, Gated STED with FCS/FLIM, Supercontinuum and 592 nm depletion laser.
Guillaume Lobet, a postdoc researcher at the University of Liege in Belgium, was frustrated by the absence of a website aggregating plant image analysis tools - so he decided to create one.
The tools listed on www.plant-image-analysis.org are not new, but it's handy to have 96 different phenotype analysis resources all in one place. Create a bookmark!
Read Lobet's Plant methods paper on this resource here: http://www.plantmethods.com/content/9/1/38/abstract.
The School of Life Sciences at Nottingham University will be hosting from April 2014 a Super Resolution Microscope facility in a Class 2 laboratory within the Centre for Biomolecular Sciences. The facility has been funded thorough an ALERT13 grant from BBSRC led by Prof Miguel Cámara with additional support from the University of Nottingham and the School of Life Sciences. The microscope purchased is the ELYRA PS.1 super resolution system from Zeiss which offers super resolution structured illumination microscopy (SR-SIM) and single molecule localisation techniques (PALM & dSTORM). The microscope stand also has an LSM 780 confocal microscope attached. Further information on the equipment is given on the Zeiss website.
The microscope will be available for Class 1 and 2 work for any researchers within the University of Nottingham and external users. Contact details and further information on bookings and training will be available on:
The Wolfson Bioimaging Facility is an established microscopy facility located in the Medical Sciences Building, University of Bristol.
"Our aim is to provide both the latest state-of-the-art equipment, as well as more routine facilities for both Light and Electron Microscopy users. We maintain an extensive suite of equipment for microscopy ranging from live cell imaging to electron tomography. We are also a fully integrated Light and Electron Microscopy Unit with considerable expertise at the interface of these techniques in correlative light-electron microscopy. We have a range of light and electron microscopes including laser scanning and spinning disk confocals, extensive widefield capability and TIRF for light microscopy. For EM, we have a FEI Tecnai 12: 120kV and FEI Tecnai 20: 200 kV transmission electron microscopes (the latter equipped with a STEM unit; suitable for cryo-EM and electron tomography, including STEM tomography), FEI Quanta 400 variable pressure scanning electron microscope and extensive EM preparation facilities.
During 2014 we will be making extensive upgrades, notably to our correlative light and electron microscopy capability (as a result of BBSRC ALERT13 funding). External users are welcome so please do get in touch."
Manager of Light Microscopy Unit: Dr. Mark Jepson, firstname.lastname@example.org
Manager of Electron Microscopy Unit: Dr. Paul Verkade: email@example.com
- Cell Biology Tool Kit
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- General Database
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