ARABIDOPSIS RESEARCH ROUND-UP
28th Aug 2013
Just one paper in this week’s Arabidopsis research round-up, but an interesting paper nevertheless!
- Lewandowska D, ten Have S, Hodge K, Tillemans V, Lamond AI and Brown JWS. Plant SILAC: Stable isotope labeling with amino acids of Arabidopsis seedlings for quantitative proteomics. PLoSONE 20 August 2013. DOI: 10.1371/journal.pone.0072207 [Open Access].
This paper, from the James Hutton Institute and the University of Dundee, describes a novel method for enabling the labeling of whole Arabidopsis seedlings with stable isotope-containing arginine and lysine. Demonstrated in seedlings exposed to salt stress, this method identified 92 up-regulated and 123 down-regulated proteins, showing that SILAC (stable isotope labeling by amino acids in cell culture) may be an important tool in analyzing the impact of mutations on quantitative protein dynamics in vivo.